Reiteration frequency of the protamine genes in rainbow trout (Salmo gairdnerii).
نویسندگان
چکیده
Protamine mRNA was isolated in a very pure form from trout testis and used as a template for the synthesis of a complementary DNA of high specific activity. The cDNA represented a full copy of the mRNA template and was used in hybridization reactions with purified trout DNA to determine the number of genes for protamines in trout testis. Our results indicate that there are less than four genes for each protamine polypeptide per the content of DNA in the male gamete and that the control of protamine synthesis in trout testis cells does not involve specific amplification of the protamine genes.
منابع مشابه
Reiteration Frequency of the Protamine Genes in Rainbow Trout (SaZmo gairdnerii)*
Protamine mRNA was isolated in a very pure form from trout testis and used as a template for the synthesis of a complementary DNA of high specific activity. The cDNA represented a full copy of the mRNA template and was used in hybridization reactions with purified trout DNA to determine the number of genes for protamines in trout testis. Our results indicate that there are less than four genes ...
متن کاملReiteration Frequency of the Protamine Genes in Rainbow Trout (SaZmo gairdnerii)*
Protamine mRNA was isolated in a very pure form from trout testis and used as a template for the synthesis of a complementary DNA of high specific activity. The cDNA represented a full copy of the mRNA template and was used in hybridization reactions with purified trout DNA to determine the number of genes for protamines in trout testis. Our results indicate that there are less than four genes ...
متن کاملObservations on Hepatic Cell Hyperplasia, Adenoma, and Hepatoma of Rainbow Trout (salmo Gairdnerii).
been reported in fish, all of them in trout (924,34, 47). In 1955 a high incidence of hepatic tumors was found in rainbow trout in Italian hatcheries (9). Interest in these neoplasms was renewed recently when hepatic tumors were discovered in large numbers of hatchery-reared rainbow trout in the United States (928,35, 592,61, 64), Italy (920), and France (3, 30). The main varieties of tumors th...
متن کاملObservations on Hepatic Cell Hyperplasia, Adenoma, and Hepatoma of Rainbow Trout (Salmo gairdnerii)4'
been reported in fish, all of them in trout (924,34, 47). In 1955 a high incidence of hepatic tumors was found in rainbow trout in Italian hatcheries (9). Interest in these neoplasms was renewed recently when hepatic tumors were discovered in large numbers of hatchery-reared rainbow trout in the United States (928,35, 592,61, 64), Italy (920), and France (3, 30). The main varieties of tumors th...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 252 22 شماره
صفحات -
تاریخ انتشار 1977